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Single cells were mechanically isolated from leafderived callus of mature Juniperus oxycedrus L. These cells divided and gave rise to callus when plated on medium containing growth regulators. Best plating efficiency was obtained on a modified Schenk and Hildebrandt medium supplemented with 0.6 µM 2,4-dichlorophenoxyacetic acid and 100 mg l −1 casein hydrolyzate. Although single-cell-derived callus showed poor morphogenic potential, both adventitious shoots and embryogenic tissuesdoi:10.1093/treephys/16.8.681 pmid:14871690 fatcat:tr5vgteo7zbjvj2q2py4qcmmja