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Defining Physiological Normoxia for Improved Translation of Cell Physiology to Animal Models and Humans
The extensive oxygen gradient between the air we breathe (PO 2~2 1 kPa) and its ultimate distribution within mitochondria (as low as~0.5-1 kPa) is testament to the efforts expended in limiting its inherent toxicity. It has long been recognized that cell culture undertaken under room air conditions falls short of replicating this protection in vitro. Despite this, difficulty in accurately determining the appropriate O 2 levels in which to culture cells, coupled with a lack of the technology todoi:10.1152/physrev.00041.2017 fatcat:mdydp2bgljaevaxqe5qttmgxci