Keywords: Resveratrol; Resveratrol Nanoemulsion (RENE); Chitosan; Hela; NF-κB and Caspase-3 Abbreviations Background: Resveratrol is a natural polyphenolic phytoalexin commonly found in fruits, grape and red wine. The objective of this study was to prepare RENE for Hela cell anticancer therapy using low chitosan and tripolyphosphate (TPP) method. Methods: In the present study resveratrol-chitosan nanoparticles was prepared made in the presences of TPP using a phase inversion method. Morphology,

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... particle size and zeta potential of RENE was then characterized. Furthermore, RENE was evaluated for its cytotoxic effect on Hela cells as well as their effect on TBARS, GSH, GST, caspase-3, NF-κB and P53. Results : RENE was showed spherical shape with mean particle size of around 49.5 ± 0.05 nm with (+15.75) negative zeta potential. The treatment of Hela cells with RENE led to a high inhibition in the Hela cell proliferation as concluded by the low IC 50 values 31.89 µg/ml. Also, RENE possess apoptotic properties via activation of the TBARS, caspase-3 and P53 protein expression as well as inhibition NF-Κb, GST and GSH levels. Conclusion: The results presented here may suggest that RENE possess anticancer and apoptotic effects on Hela cell proliferation, and therefore, can be used as new approach of pharmaceutical drugs. Also, the results clearly suggest that NF-κB, is one of the central players in the synergism of RENE and act as a regulator of caspase-3 and P53.