Syntaphilin Binds to Dynamin-1 and Inhibits Dynamin-dependent Endocytosis

Sunit Das, Claudia Gerwin, Zu-Hang Sheng
2003 Journal of Biological Chemistry  
Syntaphilin is a brain-specific syntaxin-binding partner first characterized as an inhibitor of SNARE complex formation and neurotransmitter release. Here we show that syntaphilin also binds to dynamin-1 and through this interaction inhibits dynamin-mediated endocytosis. Immunoprecipitation studies from crosslinked rat synaptosomes demonstrate that an endogenous syntaphilin-dynamin-1 complex exists independently of dynamin-1 binding to amphiphysin. Functionally, syntaphilin expression inhibits
more » ... xpression inhibits transferrin internalization in COS-7 cells. These data reveal that syntaphilin is an inhibitor of both SNARE-based fusion and dynaminmediated endocytosis.
doi:10.1074/jbc.m304851200 pmid:12896979 fatcat:dqfrgs4efjhqvcx75b4ssu6wyu