Characterization of a novel activation antigen on porcine lymphocytes recognized by monoclonal antibody 5A6/8

Nieves Doménech, Rosario Bullido, Belén Álvarez, Emilia Campos, María-Pilar Rodríguez-Carreño, Gómez del Moral, Fernando Alonso, Ángel Ezquerra, Javier Domínguez
2004 Veterinary Research  
In this report, we describe the characterization of a novel activation antigen on porcine lymphocytes recognized by mAb 5A6/8. This antigen was detected on B and T cells 24 h after treatment with various stimuli. It was also found on alveolar macrophages, and at low levels on untreated monocytes. MAb 5A6/8 precipitated two bands of 45 and 50 kDa under non-reducing conditions, and of 22 and 28 kDa under reducing conditions. The cellular distribution, expression kinetics and/or molecular size of
more » ... molecular size of the 5A6/8 antigen differ from those of other known lymphocyte activation antigens. MAb 5A6/8 was able to inhibit lymphocyte proliferative responses driven by different stimuli, suggesting a role for this molecule in the events that lead to lymphocyte activation. activation antigen / lymphocyte / monoclonal antibody / monocyte / pig
doi:10.1051/vetres:2004017 pmid:15210082 fatcat:im2ziepqzjccthvdo5evy4zpdq