Isolation and characterization of novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus (Coleoptera: Nitidulidae)

Oddmund KLEVEN, Anders ENDRESTOL
2016 European Journal of Entomology  
Here we describe 16 novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus, which were identifi ed using a next-generation sequencing approach. In 40 dragonhead sap beetles from a population in southern Norway, we found 2-4 alleles per locus. Expected heterozygosity ranged from 0.18-0.65. Three of the loci showed signifi cant deviation from Hardy-Weinberg equilibrium. After correcting for multiple tests, one pairwise locus combination displayed signifi cant
more » ... inkage disequilibrium. These novel microsatellite loci will be useful for future population structure, genetic diversity and conservation genetic studies of the dragonhead sap beetle.
doi:10.14411/eje.2016.071 fatcat:2jae6fvpufcs3kmp7tq7kteunm