DEVELOPMENT AND VALIDATION OF VISIBLE METHOD FOR ESTIMATION OF AMPICLLIN IN BULK FORMULATION

Chavi Dagar
2017 World Journal of Pharmaceutical Research  
A simple, sensitive, accurate, precise and economical visible Spectrophotometric method was developed and validated for the estimation of Ampicillin in Bulk form. The method is based on the reaction of Ampicillin with FC Reagent [Folin Ciocalteu] in the presence of 10% Sodium Carbonate giving greenish blue colour chromogen which shows maximum absorbance at 720nm against reagent blank. The Chromogen obeyed Beer's law in the concentration range of 10-50 µg/ml for Ampicillin. The results of the
more » ... e results of the analysis have been validated statistically and recovery studies.
doi:10.20959/wjpr20176-8594 fatcat:qdrphgkeuvg3jfku3e4vd5b4i4