A Proton Wire and Water Channel Revealed in the Crystal Structure of Isatin Hydrolase

Kaare Bjerregaard-Andersen, Theis Sommer, Jan K. Jensen, Bjarne Jochimsen, Michael Etzerodt, J. Preben Morth
2014 Journal of Biological Chemistry  
IMBG), University of Aarhus, as part of a double degree agreement. The thesis is divided into two parts. Part I describes my work on the crystallization and biophysical characterization of the N-terminal cytoplasmic domains of the membrane proteins NCBE. Part II describes my work on structure determination, biophysical characterization and biotechnological application of the enzyme isatin hydrolase. The cloning and purification of isatin hydrolase described in section 16.1.1, "Cloning,
more » ... n and purification of IH-b-wt and IH-b-S225C" and paper II was carried out by Anette Kjems and Michael Etzerodt at Aarhus University, Denmark. The measurement of thermostability of IH-b-wt and IH-b-S225C described in the section 16.1.5 "Thermostability determination by circular dichroism" and paper II was carried out
doi:10.1074/jbc.m114.568824 pmid:24917679 pmcid:PMC4118100 fatcat:hpcxamzg2zdafdmnnkjiai42gi