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Abstract-IntroductionThe recently developed ultrastructure expansion microscopy (U-ExM) technique allows to increase the spatial resolution within a cell or tissue for microscopic imaging through the physical expansion of the sample. In this study we validate the use of U-ExM inTrypanosoma bruceiby visualizing the nucleus and kDNA as well as proteins of the cytoskeleton, the basal body, the mitochondrion and the ER.T. bruceiis a unicellular flagellated protozoan parasite and the causative agentdoi:10.1101/2021.04.20.440568 fatcat:tsbqtfdqavferok4jiehyblade