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We present a checklist for reptiles and amphibians of the Reserva Extrativista do Rio Gregório, at the upper Juruá River basin, in the southwest Brazilian Amazonia. Using time-constrained searches, pitfall traps, vocalization, and accidental sightings, we recorded 84 species: 46 amphibians and 38 reptiles. Although analyses suggest still higher diversity, considering the short sampling time, relatively high species richness was documented, which reveals the relevance of this region fordoi:10.15560/8.3.360 fatcat:iypr6ykqerebhdoxgeahtxbdia