Prospects for photostimulation of nisin biosynthesis
IOP Conference Series: Earth and Environment
The purpose of the article was to study the effect of blue spectrum light on the nisin producer Lactobacillus lactis. It was found that photostimulation of a Lactobacillus Lactis culture with blue spectrum light (435-470 nm) with a light flux intensity of 1800 mcd for 50-60 minutes positively affects the activity of nisin (increases by 60.1%), while the titer of the Lactobacillus culture lactis is 2 times higher. At the same time, there is a slight decrease in nisin titer after 24 hours of
... ation, which is explained by the peculiarities of nisin biosynthesis: nisin is less active at the beginning of biosynthesis; during the period of the exponential growth phase, an increase in the biosynthesis of nisin is noted; the greatest activity of nisin is noted at the beginning of the stationary phase; nisin synthesis is reduced in the middle of the stationary phase of the cells; self-regulation of nisin synthesis (increased nisin synthesis leads to increased competition for metabolites of the substrate and energy material, nisin molecules act as an external factor that regulates synthesis). Light treatment of the nisin producer Lactobacillus lactis increases its resistance to oxidative stress and enhances its viability. As a result of studies during storage of the Lactobacillus lactis culture in skimmed milk, the positive effect of light on the high preservation of nisin activity was proved. Thus, photostimulation of the bioproducer of nisin -Lactobacillus Lactis culture with blue light with a light flux intensity of 1800 mcd for 50-60 minutes has a positive effect on its viability and allows us to recommend the use of blue light to increase nisin production.