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Chlamydia trachomatis one of the main causes of pelvic inflammatory diseases which leading to infertility condition especially in women. In this study, 63 vaginal swab specimens from infertile women in Nasiriyah city (south of Iraq) were collected and analyzed by polymerase chain reaction (PCR) by using specific bacterial DNA primer and the positive results correlate in relation to age and types of infertility (primary and secondary). The current study showed that only 9.5% of the samples haddoi:10.32792/utq/utj/vol14/1/1 fatcat:ppsbiwglozfr7mgmifwzgex6ee