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During ethanol production, the ratio of various sources of starch-hydrolyzing enzymes significantly influences hydrolysis efficiency in a very high gravity (VHG) system. In this study, the enzyme matching approach was proposed to optimize liquefaction and saccharification yield of cassava root and cassava starch. For fresh cassava root, the synergistic effect of using Techzyme Q-Add enzyme (93 °C pH 5.6) for liquefaction and GC 147 enzyme (61.5 °C pH 4.2) for saccharification achieved thedoi:10.15376/biores.13.2.2304-2327 fatcat:pexh3x2aj5gezomrbeoff3r37i