The systematic eco-based approach of silver nanoparticles (Ag-NPs) have developed using (Kappaphycus alvarezii) has tremendous advantages in drug discovery. The sponge was extracted for biosynthesis of Ag-NPs from the aqueous silver nitrate (AgNo 3) solution. The Ag-NPs was characterized by UV-Vis, scanning electron microscopy (SEM). The Ag-NPs surface plasmon exhibited maximum at 445 nm. SEM show the surface morphology of Ag-NPs. The antibacterial activity was performed Escherichia coli and

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... phylococcus aureus. This study concludes that the Ag-NPs synthesized from K. alvarezii has great potential compound to target against pathogenic microorganisms.