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Differential Regulation of Ota and Otb, Two Primary Glycine Betaine Transporters in the Methanogenic Archaeon Methanosarcina mazei Gö1
Journal of Molecular Microbiology and Biotechnology
Methanogenic archaea accumulate glycine betaine in response to hypersalinity, but the regulation of proteins involved, their mechanism of activation and regulation of the corresponding genes are largely unknown. Methanosarcina mazei differs from most other methanoarchaea in having two gene clusters both encoding a potential glycine betaine transporter, Ota and Otb. Western blot as well as quantitative real-time PCR revealed that Otb is not regulated by osmolarity. On the other hand, cellulardoi:10.1159/000108656 pmid:17878709 fatcat:membucvohjcclebuydpox2pe7a