The 5-hydroxytryptaminergic Neurons Locating in the Dorsal Raphe Nucleus and Different Receptors of 5-HT Were Implicated in Mediating the Arousal from Sevoflurane Anesthesia [article]

HaiXiang Ma, Qian Yu, XiTing Lian, YuLing Wang, Qing Xu, Yue Shen, HongHai Zhang
2022 bioRxiv   pre-print
In order to explore the mechanism of general anesthesia emergence,based on the common clinical phenomenon--delayed emergency, we explore the role of 5-HT neurons in the dorsal raphe nucleus in promoting sevoflurane arousal in mice model of general anesthesia. In this study, C57BL/6J male mice were selected to specifically activate or inhibit DR 5-HT neurons and different 5-HT receptors by intraperitoneal, lateral ventricle or intranuclear injection and optogenetics during the sevoflurane
more » ... sia, record and observe the anesthesia induction and emergence time of mice. Through intraventricular infusion and intranuclear microinjection of serotonin and 5-HT receptor agonists or antagonists, study shows that serotonin and 5-HT 1A and 2A/C receptor, especially 5-HT 1A receptor, are involved in the regulation of awakening delay mediated by DRN 5-HT neurons. This can provides a reliable theoretical basis and intervention target for clinical prevention of delayed emergency. Key words: general anesthesia, sevoflurane, delayed emergency, dorsal raphe, 5-Hydroxytryptamine
doi:10.1101/2022.05.22.492958 fatcat:pzkouevc45bbfb2d7ptyspe4di