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In order to explore the mechanism of general anesthesia emergence,based on the common clinical phenomenon--delayed emergency, we explore the role of 5-HT neurons in the dorsal raphe nucleus in promoting sevoflurane arousal in mice model of general anesthesia. In this study, C57BL/6J male mice were selected to specifically activate or inhibit DR 5-HT neurons and different 5-HT receptors by intraperitoneal, lateral ventricle or intranuclear injection and optogenetics during the sevofluranedoi:10.1101/2022.05.22.492958 fatcat:pzkouevc45bbfb2d7ptyspe4di