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Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm
2017
BMC Genomics
The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the introduction of wanted mutations. Unfortunately, it has been reported repeatedly that the sgRNA can also guide Cas9 to off-target sites where the DNA sequence is homologous to sgRNA. Results: Using human genome and
doi:10.1186/s12864-017-4225-1
pmid:29219081
pmcid:PMC5773898
fatcat:pppq6kdm5jg2bfza3mjikxletm