Oral and intranasal administration of somatostatin DNA vaccine mediated by attenuated Salmonella Enterica Serovar Typhimurium to promote growth of piglets

X. B. Liu, A. X. Liang, X. G. Feng, A. Z. Guo, C. Y. Ke, S. J. Zhang, L. G. Yang
2011 Animal  
An attenuated strain ofSalmonella typhimuriumhas been used as a carrier for oral and intranasal genetic immunization. Here, we evaluate the efficacy of a vaccine strain ofS. typhimurium.CSO22 (pGM-CSF/SS, plasmid granulocyte-macrophage colony-stimulating factor/somatostatin) expressing two copies ofSSgenes. A total of 115 piglets, aged 2 months old, were either orally or intranasally immunized against the vaccine strain CSO22 (pGM-CSF/SS) with three dosages (5 × 1010colony forming units (CFU),
more » ... rming units (CFU), 5 × 109CFU and 5 × 108CFU). For oral immunization, the specific anti-SS antibodies were detected in the immunized piglets. The levels of SS antibodies in the high-dose immunized group (5 × 1010CFU) were significantly higher than that in the phosphate buffered saline immunized group (P< 0.01) and 40% of animals were positive in SS antibodies in the high-dose immunized group. Moreover, the weight gain of the high-dose group was increased by 20.86%, 10.26% and 15.30% during 4, 8 and 12 weeks, respectively, after immunization in comparison to the control. For intranasal immunization, the growth of the low-dose group was increased by 10.23% in the whole test period (12 weeks). In conclusion, our results suggest that the recombinant strain could elicit anti-SS antibodies and improve the growth performance of immunized piglets, and that the oral immunization program is better than the intranasal program.
doi:10.1017/s1751731111000231 pmid:22440175 fatcat:5axysjvu2nfenkco3fxs67aeuq