Proceedings of "The 16th International and Iranian Congress of Microbiology
Name Sarvenaz, Bigham Soostani, Rahman Patimar, Zarei Darki, Esa Jorjani, Kourosh Sayehmiri, Milad Azami, Zahra Darvishi, Sasan Nikpay, Milad Borji, Pariya Ahmadi Balootaki, Mansour Amin
(+746 others)
2015
Iranian J Publ Health
unpublished
Cyanobacteria as photosynthetic organisms have a main effect on life and productivity of the aquatic ecosystems. The long-term studies on the identification, distribution and diversity of Cyanobacteria is crucial in the Caspian Sea. Methods: Four transects were determined parallel to the Sabzehrud River, the Lavij River, Noor River, and the Department of Marine Biology of Tarbiat Modares University from 2014 to 2015. Samples were seasonally collected from three stations in each transect by a
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... nkton net with a mesh size of 55 and the Ruthner's bathometer. Standard hydrobiological and phycology approaches were used for identification and totaling abundance of cyanobacteria. Results: The most number of cyanobacteria was observed in station A1 with number of 1062500 N/L during summer season. Genera Chroococcus, Gloeocapsa, Merismopedia, Microcystis belong to unicellular taxa and Anabaena, Anabaenopsis, Nostoc, Oscillatoria, Phormidium were identified as the multicellular genera. Anabaenopsis nadsonii was observed as the most distributed species. Conclusion: The result showed that cyanobacteria abundance was significantly different among stations and all seasons. The reported blue-green algae also have no same frequency among stations inside each transect (Test Chi-Square, P <0.05). Background: This study aimed to investigate the prevalence of hepatitis B infection in health care workers in Iran as a systematic review and meta-analysis study. Method: To obtain corresponding Persian and English documentations, Iranmedex, SID, Medlib, Scopus, PubMed, Science Direct, Cochrane, Embase, Web of Science, Springer, Online Library Wiley and also Google Scholar in chronological were searched with no time limit to 2015 by two independent researchers using standard key words. Criteria for diagnosis of hepatitis B infection, HBsAg (+) were considered. Data manipulation and statistical analyses were performed random effect model by using Stata Ver.11.1. Results: In the 20 eligible studies, the 4280 individuals were been evaluated. The prevalence of hepatitis B infection in health care workers of Iran 0.4 %( 95% CI: 0.1-0.6) was estimated. The minimum and maximum percent were related to the center country (0.3%) and West (4.1%), respectively. The prevalence of HBcAb in health care workers of 5.9 %( 95% CI: 4.2-7.6) was estimated. Conclusion: The prevalence of hepatitis B among health care workers in Iran to our expectation was lower than the Iranian general population. Background: Erysipelothrix is long and thin facultative anaerobic Gram-positive, nonsporulating, intracellular rod-shaped bacterium, and is widely distributed in nature. Methods: One hundred fifty samples were taken from slaughterhouse workers, slaughter, fishermen, fish handlers and fishes, liver and heart of sheep and calf by swabbing method. Detection and distribution of Erysipelothrix rhusiopathiae in all specimens were performed using phenotypical and PCR amplification methods. Results: Out of 150 samples, 12 positive isolates were recovered by phenotypical properties related to Erysipelothrix rhusiopathiae, that they were also PCR positive. According to PCR analysis, 8 more cases (5.33%) among 134 negative cultures were found as the strain of Erysipelothrix rhusiopathiae. In general, by using molecular method 20 (13.3%) samples were observed as E. rhusiopathiae. All confirmed isolated bacteria by PCR were highly sensitive to Penicillin, Ciprofloxacin, Impenem, Erythromycin. All of the above mentioned isolates were resistant to Gentamycin and Neomycin. Conclusion: Erysipelothrix rhusiopathiae is widely distributed on seafood's and present as commensally pathogen in nature and animals. Infection with this microorganism must be emphasized because it is a rare organism that is causative of severe infection including infectious endocarditis, polyarthritis following localized infections. Background: Rotavirus is the leading cause of severe diarrhea in children with a worldwide distribution. The purpose of this investigation was to determine if green tea (Camellia Sinensis) extract, could inhibit rotavirus infection in cultured BS-C-1 (monkey kidney epithelial) cells. Methods: Effects of green tea extract on BS-C-1 cultured cells and rotavirus was assessed by using cell viability and proliferation assays. After establishing the maximum noncytotoxic concentration of green tea extract, BS-C-1cells and rotavirus particles were treated with varying concentrations of green tea extract. BS-C-1 cells were infected with rotavirus and the effect of infectivity was determined by TCID50 and MTT assays. Green tea solutions with concentrations of 50, 500, 1000 μg/mL in water, were allowed to react with simian rotavirus SA11, and the inhibition of tea extract was quantified using the TCID50 assay. Results: Green tea extract was not cytotoxic to BS-C-1 cells, as confirmed by cell viability and proliferation assays, in which green tea extract treat group paralleled the positive control group. We found that extracts from green tea reduced viral infectivity in more than 4-log scale against rotavirus tested, is showing strong antiviral effects against rotavirus. Conclusion: Green tea extract is not cytotoxic and can reduce or block the production of infectious rotavirus in cultured BS-C-1 cells. The findings indicate that green tea extract has the potential to be developed as a safe, therapeutic antiviral agent to prevent the spread of rotavirus.
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