Purpose: The aim of this study was to evaluate the effect of oral microorganism on surface topography of acrylic resin denture base material. Materials and methods: Fifty rectangular plates of dimensions 30mm x 20mm x 1 mm were fabricated with heat polymerizing acrylic resin denture base material. an area of 10mm x 10 mm in the center of each acrylic plate was marked for evaluation of surface topography by Laser Triangulation Sensor.The plates were then sterilized by immersion in 2 %

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... yde for 30 minutes, and placed in sterilized containers. saliva sample were collected from 40 healthy edentulous people of 40 to 60 years in age wearing acrylic complete denture. 0.1 ml of saliva was immediately poured on central portion of each were placed in incubator at 37 degree centigrade for 72 hr. after 72 hr each plate was inspected for bacterial colonies. Results: The mean value of difference of mean surface roughness value (ra) of acrylic plates before culture and after culture of microorganism is 2.13343 with overall standard deviation of 0.9964, and standard error 0.1821. Conclusion: The acrylic resin plates showed some amount of roughness even after polishing (mean surface roughness value 'Ra') before culture of microorganism on them. The acrylic resin plate showed an increase in mean surface roughness after culture of microorganism. Thus this roughness will deteriorate the denture by the time, even after the use of latest denture cleanser and other auxiliaries, by offering more plaque and microorganism accumulation and cycle go on.