IL-33 induces Th17-mediated airway inflammation via mast cells in ovalbumin-challenged mice

Kyung-Ah Cho, Jee Won Suh, Jung Ho Sohn, Jung Won Park, Hyejin Lee, JiHee Lee Kang, So-Youn Woo, Young Joo Cho
2012 American Journal of Physiology - Lung cellular and Molecular Physiology  
YJ. IL-33 induces Th17-mediated airway inflammation via mast cells in ovalbumin-challenged mice. Allergic asthma is characterized by infiltration of eosinophils, elevated Th2 cytokine levels, airway hyperresponsiveness, and IgE. In addition to eosinophils, mast cells, and basophils, a variety of cytokines are also involved in the development of allergic asthma. The pivotal role of eosinophils in the progression of the disease has been a subject of controversy. To determine the role of
more » ... s in the progression of airway inflammation, we sensitized and challenged BALB/c wild-type (WT) mice and eosinophil-deficient ⌬dblGATA mice with ovalbumin (OVA) and analyzed different aspects of inflammation. We observed increased eosinophil levels and a Th2-dominant response in OVA-challenged WT mice. In contrast, eosinophil-deficient ⌬dblGATA mice displayed an increased proportion of mast cells and a Th17-biased response following OVA inhalation. Notably, the levels of IL-33, an important cytokine responsible for Th2 immune deviation, were not different between WT and eosinophil-deficient mice. We also demonstrated that mast cells induced Th17-differentiation via IL-33/ST2 stimulation in vitro. These results indicate that eosinophils are not essential for the development of allergic asthma and that mast cells can skew the immune reaction predominantly toward Th17 responses via IL-33 stimulation.
doi:10.1152/ajplung.00252.2011 pmid:22180658 fatcat:bh4osvxcxzey5mvs3csji5o4wm