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Additional file 1. Table S1. Sugar yields obtained from different pretreatment strategies. Table S2. Percent mass loss obtained after different pretreatments. Figure S1. Water retention value (WRV) for untreated biomass and alkaline pre-extracted biomass. Figure S2. Enzymatic hydrolysis inhibition studies using Avicel as a substrate. Figure S3. Partial 2D HSQC NMR spectra of (A) lignin from untreated, debarked whole poplar, (B) solubilized lignin from reference case Cu-AHP pretreated poplar,doi:10.6084/m9.figshare.c.3632408_d1 fatcat:okxea7b3rvahhngluemm2twcui