Effect of Using Different Compensation of Growth Regulators on Hypocotyls Callus Intiation and Differentiate of Medicago Sativa

Raghad Nawaf Al-zaidy, Najwa Ibraheem Khalil Al-Barhawi, Abdullah Najim Abdullah Al-niemi
2018 Kirkuk University Journal-Scientific Studies  
The effect of some growth regulators on intiation ,grwth and differtiate of Alfalfa callus was studied .The search results showed a distinction MS media supplemented with growth regulators such as Kin ( 0.1 mg\l) +2,4-D( 1.0 mg \l) were distinguished in promoting callus formation from leaves and roots of Medicago sativa plants at ratio 90 and 100% successively. On the other hand the medium Kin (2.0mg\L) +2,4-D (2.0mg\L) formed callus from hypocotyl stem of seedlings in addition to formation of
more » ... on to formation of regeneration branches at number reached to 24 and of ratio 20% in one period.
doi:10.32894/kujss.2018.145760 fatcat:ttdyrya7efbsbolconrhg7jwwu