Simultaneous determination of pyrrolizidine alkaloids in food supplements of herbal origin using liquid chromatography tandem mass spectrometry

Son Tran Cao, Tien Bui Cao, Thao Dang Phuong, Truong Nguyen Xuan, To Quynh Cung Thi, Hong Hao Le Thi, National Institute for Food Control, National Institute for Food Control, Hanoi University of Pharmacy, Vietnam Food Administration, Hanoi University of Sicence and Technology, National Institute for Food Control
2019 Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam  
A simple and accurate method has been developed for the simultaneous determination of four pyrrolizidine alkaloids (PAs) including echimidine, senecionine, jacobine and intermidine in food supplements of plant origin. The instrumental conditions including the use of TSK-Gel column for the separation and an MS/MS detector in positive ESI source, multi-reactive monitoring (MRM) mode for quantitation were optimized. The method has fully validated and meets the performance
more » ... equirement of AOAC with good specificity. The linearity varies from 1.0 µg/kg to 40 µg/kg, the LOD was of 0.3 µg/kg; the repeatability and recovery range from 3.6% to 7.5% and 88.7% to 117%, respectively. The method was applied to determine PAs in 90 food supplement samples, of which 26 samples (28.8%) contain PAs.
doi:10.47866/2615-9252/vjfc.58 fatcat:gm2oa7r6nvfkhdd4wo75vojweq