Specific Hypersensitive Response–Associated Recognition of New Apoplastic Effectors from Cladosporium fulvum in Wild Tomato

Carl H. Mesarich, Bilal Ӧkmen, Hanna Rovenich, Scott A. Griffiths, Changchun Wang, Mansoor Karimi Jashni, Aleksandar Mihajlovski, Jérôme Collemare, Lukas Hunziker, Cecilia H. Deng, Ate van der Burgt, Henriek G. Beenen (+3 others)
2018 Molecular Plant-Microbe Interactions  
35 Tomato leaf mould disease is caused by the biotrophic fungus Cladosporium fulvum. During 36 infection, C. fulvum produces extracellular small secreted protein (SSP) effectors that 37 function to promote colonization of the leaf apoplast. Resistance to the disease is governed by 38 Cf immune receptor genes that encode receptor-like proteins (RLPs). These RLPs recognize 39 specific SSP effectors to initiate a hypersensitive response (HR) that renders the pathogen 40 avirulent. C. fulvum
more » ... t. C. fulvum strains capable of overcoming one or more of all cloned Cf genes have 41 now emerged. To combat these strains, new Cf genes are required. An effectoromics 42 approach was employed to identify wild tomato accessions carrying new Cf genes. 43 Proteomics and transcriptome sequencing were first used to identify 70 apoplastic in planta-44 induced C. fulvum SSPs. Based on sequence homology, 61 of these SSPs were novel or 45 lacked known functional domains. Seven, however, had predicted structural homology to 46 antimicrobial proteins, suggesting a possible role in mediating antagonistic microbe-microbe 47 interactions in planta. Wild tomato accessions were then screened for HR-associated 48 recognition of 41 SSPs using the Potato virus X-based transient expression system. Nine 49 SSPs were recognized by one or more accessions, suggesting that these plants carry new Cf 50 genes available for incorporation into cultivated tomato. 51 52
doi:10.1094/mpmi-05-17-0114-fi pmid:29144204 fatcat:jxaepx57ofcorle52lrbn55d54