Micromechanical Behavior of Potato Tissue by Scanning Electron Microscopy: Effect of Storage Time, Impact Energy and Curvature Radius of Impact Location

S Youneji, E Ahmadi, A Alavi Nia
2018 Journal of Agricultural Machinery  
Introduction The mechanical impacts occur mainly during harvesting and post-harvesting operations, lead to the breaking of cell membranes in cellular structure that dependS on impact intensity. Furthermore, turgor pressure of potato tissue is influenced by the micromechanical and the physiological changes in the storage duration. Micromechanical changes of potato tissue due to the mechanical impact need to be monitored by microscopic images during storage. Scanning electron microscopy (SEM) is
more » ... icroscopy (SEM) is a high-resolution technique used to investigate the micromechanical behavior of potato tissue. Materials and Methods Potato samples ('Sante' cultivar), were stored at 5 ± 0.5°C and 85% relative humidity for 16 weeks. By 2-week intervals, potatoes were removed from the storage and then the impact test was done. Experimental factors were impacted energy at three levels of control (no impact was done), impact energy 1 (0.031 ± 0.002 J) and impact energy 2 (0.320± 0.020 J) and the radius of curvature at two levels of (35 and 45 mm). Water content was measured by drying thin slab samples in an oven at 70°C to a constant weight. The cell turgor pressure of potato tissue at 2-week intervals was estimated from the linear regression between turgor values of each mannitol solution (0–0.6 M) and relative volume change. The microstructural changes of impact location on the potato tubers were analyzed by SEM images at 2-week intervals during storage period. The surface and depth sections cutting from the impact location were immediately immersed in 2.5% glutaraldehyde in 0.1 M sodium phosphate buffer (2 h) at 4± 0.5°C. The specimens were then rinsed 3 × 10 min in 0.1 M sodium phosphate buffer (pH 7.2), and dehydrated through an ethanol series, 25, 50, 70, 90 and 100% dry, 15 min each step, 2 × 100%. In this study the HMDS as a high-quality chemical drying was investigated. The sample preparation for SEM observation then followed by chemical drying via HMDS, under a laboratory hood overnight. Analysis of variance test based on completel [...]
doi:10.22067/jam.v8i2.62599 doaj:9fcd39bd08064d4283904a7f993e1572 fatcat:uzpgxi2bejdynptechcdygehru