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Estimating RNA structure chemical probing reactivities from reverse transcriptase stops and mutations
[article]
2018
bioRxiv
pre-print
Chemical probing experiments interrogate RNA structures by creating covalent adducts on RNA molecules in structure-dependent patterns. Adduct positions are then detected through conversion of the modified RNAs into complementary DNA (cDNA) by reverse transcription (RT) as either stops (RT-stops) or mutations (RT-mutations). Statistical analysis of the frequencies of RT-stops and RT-mutations can then be used to estimate a measure of chemical probing reactivity at each nucleotide of an RNA,
doi:10.1101/292532
fatcat:xvrwks52wvbqldp6lsrkjis2k4