Soy Meal Waste Extract as Cultivation Medium for Production of Extracellular α-Galactosidase from the FungusHumicola Lutea120–5

L. Nacheva, P. Aleksieva, E. Bratovanova, I. Stoineva, B. Yakimova, B. Tchorbanov
2008 Biotechnology & Biotechnological Equipment  
Soy meal extract presenting a waste product from soy protein concentrate manufacture was applied as cultivation medium for the production of extracellular α-galactosidase in submerged fermentation of the fi lamentous fungus Humicola lutea 120-5. Maximum enzyme activity (2000 U.l -1 ) was produced when the soy meal extract containing 5% dry substances (~1% raffi nose oligosaccharides) was used at 120th h cultivation and 35°C. Chromatography on Sephadex G-100 and DEAE-cellulose resulted in a
more » ... resulted in a purifi ed α-galactosidase fraction with specifi c enzyme activity of 37 U.mg -1 . The optimum conditions for the enzyme reaction were pH 4.0 to 4.5 at 50°C. The partially purifi ed enzyme was stable up to 40°C at pH 2.0 to 7.5. The crude enzyme preparation readily hydrolyzes raffi nose according HPLC analysis, but no activity against disaccharides as sucrose and melibiose was observed. The soy meal waste extract can be recommended as a suitable medium for the industrial cultivation of strains producing α-galactosidase for food and fodder purposes.
doi:10.1080/13102818.2008.10817544 fatcat:2qbyg2wgizanfaav23f33vi4ui