Plant Kinases [book]

Nico Dissmeyer, Arp Schnittger
2011 Msphere  
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more » ... etary rights. Cover illustration: The title image is a collage of three different Arabidopsis thaliana plants. The endogenous activity of one of the major cell cycle kinases cyclin-dependent kinase A;1 (CDKA;1) increases from left to right. The two left individuals are cdka;1 homozygous null mutants that were partially complemented by phosphorylation-site substituted CDKA;1 variants and are compared to a wild type plant of the accession Columbia-0 on the right. The phosphosite mutants were expressed from the endogenous CDKA;1 promotor. The CDKA;1 variant on the left carries the double phospho-mimetic substitution T14D/Y15E in the ATP-binding pocket (P-loop) and that one in the center the single amino acid substitution T161D in the activation loop (T-loop). Both domains of CDKA;1 are required for catalysis and substitutions lead to a tremendous reduction in kinase activity. For reference, see Dissmeyer N.
doi:10.1007/978-1-61779-264-9 fatcat:ky4xaqh3uvawzeqdmgjhirfpmi