Faculty of 1000 evaluation for Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes [dataset]

Humphrey Yao, Ivraym Barsoum
2010 F1000 - Post-publication peer review of the biomedical literature   unpublished
Granulosa cells of mammalian Graafian follicles maintain oocytes in meiotic arrest, which prevents the precocious maturation. We show that mouse mural granulosa cells, which line the follicle wall, express natriuretic peptide precursor type C, Nppc, mRNA while cumulus cells surrounding oocytes express mRNA of the NPPC receptor NPR2, a guanylyl cyclase. NPPC elevated cGMP levels in cumulus cells and oocytes and inhibited meiotic resumption in vitro. Meiotic arrest was not sustained in most
more » ... an follicles of Nppc or Npr2 mutant mice, and meiosis resumed precociously. Oocyte-derived paracrine factors promoted cumulus cell expression of Npr2 mRNA. Therefore, the granulosa cell ligand NPPC and its receptor NPR2 in cumulus cells prevent precocious meiotic maturation, which is critical for maturation and ovulation synchrony and for normal female fertility.
doi:10.3410/f.5666956.7253054 fatcat:l3acchmzzrec7ef4rwtgjscna4