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Quantitative Imaging of Nitrogen Fixation by Individual Bacteria Within Animal Cells
2007
Science
Materials and Methods Sample Preparation: Cells of T. turnerae T7902 were grown as previously described (S1). The headspace gas was replaced with 0.5 ml of 15 N 2 (98%). The cells were incubated at room temperature for up to 96 hr (approx. eight doublings). Cells of E. faecalis were also incubated in 15 N 2 as above for 2.5 hr (> 4 doublings) at 37°C in tryptic soy broth medium (Bacto). Specimens of L. pedicellatus were raised as previously described (S2). Specimens residing in wood were
doi:10.1126/science.1145557
pmid:17872448
fatcat:wwwutlf6qffrjopgoh2i6tbivu