A Toxic Substance in Carcinomatous Ascites

Hirosi Tiba, Isao Takaku, Genji Kikuchi
1953 Tohoku journal of experimental medicine  
A further study on the toxic substance in a preceding work1) revealed that it is 1) heat-unstable, 2) soluble in methanol, ethanol, butanol and acetone, and 3) precipitable from water with lead acetate but not with baryta. It migrated to the anode on electrodialysis as already described by Aikawa.' > Moreover, an impure preparation of its sodium salt, ob tained from an acidic fraction of a carcinomatous ascitic fluid through successive barium and lead salts, analyzed: N 1.8%, amino-N 0.4% and
more » ... h 44.6%, suggesting a non-nitrogenous, low-molecular acid for the pure substance. Autopsy findings of the mice killed by injection of the substance (hyperemia and hemorrhage of internal organs) and the effect of it on the beat of a frog heart (depression of the heart) will be also de scribed below, together with some additional observations. EXPERIMENTAL
doi:10.1620/tjem.59.79 fatcat:4r7uqowl5ra37gfoqxsxjoioy4