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Determination of the Disulfide Bond Arrangement of Dengue Virus NS1 Protein
Journal of Biological Chemistry
The 12 half-cystines of NS1 proteins are absolutely conserved among flaviviruses, suggesting their importance to the structure and function of these proteins. In the present study, peptides from recombinant Dengue-2 virus NS1 were produced by tryptic digestion in 100% H 2 16 O, peptic digestion in 50% H 2 18 O, thermolytic digestion in 50% H 2 18 O, or combinations of these digestion conditions. Peptides were separated by size exclusion and/or reverse phase high performance liquiddoi:10.1074/jbc.m312907200 pmid:14981082 fatcat:c2mkomaqxrcsvjtntnbplmbwxe