Extraction and Characterization of Chitosan by Simple Technique from Mud Crabs

N. A. Musmade Lalit Mahatma
2021 International Journal of Current Microbiology and Applied Sciences  
Chitosan is a naturally available biopolymer. It occurs as a component of crustacean shells, insect exoskeletons, fungal cell walls and plankton. In this work, chitin was extracted from mud crab shells waste. Chitosan, a useful biopolymer, was obtained from pulverized shells of crabs via demineralization, deproteination and de-acetylation. These steps were crucial for the elimination of calcium carbonate and other minerals as well as protein which are present in the shells. In this study, the
more » ... emical compositions of crab shells waste were analyzed by Fourier transform infrared spectroscopy (FTIR). From the 200 g exoskeleton of mud crab, 52.46 g (26.23%) chitin was extracted. Out of the 52.46 g chitin, 38.68 g (73.73%) chitosan was obtained.
doi:10.20546/ijcmas.2021.1006.055 fatcat:n3gk4d2lcjbljebch5yigsog5i