TAM METİN SÖZLÜ SUNUMLAR / FULL TEXT ORAL PRESENTATIONS

2019 Turk biyokimya dergisi = Turkish journal of biochemistry  
Objective: Hyperglycemia complications have negative effect on the movement system like all other systems in patients with diabetic neuropathy. In these patients, it is necessary to get a real clinical diagnosis and it is important to understand the relationship between movement system and hyperglycemia. Our purpose was to investigate the relationship between hyperglycemia values and changes in the movement system in patients with diabetic peripheral neuropathy. Materials and Method: We
more » ... 33 patients aged between 45-76 with Type 2 diabetic neuropathy. Patient's demographic characteristics and duration of disease were recorded. Measurements related with the movement system are performed with biosensoric Biodex Balance SD balance systems. Adaptation time to gravity change and postural sway parameters were examined in the movement system. Results: The mean age of the patients was 60.72± 7.05(48-76) years. There was a positive correlation between adaptation time to gravity change and age (r:0.445). In addition, there was a positive relationship between adaptation time to gravity change and body mass index measurements (r:0.429). There wasn't found any relationship between hyperglycaemia parameters (HbA1c and fasting blood glucose) and movement measurements. Conclusion: There wasn't found direct relationship between hyperglycemia and movement system. Conflict of interest: Authors do not have a conflict of interest Öz Amaç: Diyabetik nöropatisi olan olgularda hiperglisemi kaynaklı komplikasyonlar tüm diğer sistemler gibi hareket sistemini de olumsuz etkilemektedir. Bu hastalarda gerçek klinik tanıyı almak ve hareket sistemi ile hiperglisemi ilişkisini anlamak gerekmektedir. Çalışmanın amacı; diyabetik nöropatili hastalarda hiperglisemi değerleri ile hareket sistemindeki değişiklikler arasındaki ilişkiyi incelemektir. Gereç ve Yöntem: Araştırmamıza 45-76 yaş aralığında olan Tip 2 diyabetik nöropatisi olan 33 hasta dahil edilmiştir. Hastaların diyabet süreleri ve demografik özellikleri kaydedilmiştir. Hareket sistemi ile ilgili olan ölçümleri biyosensörlü Biodex Balance SD denge sistemleri ile yapılmıştır. Hareket sisteminde gravite değişikliğine uyum süresi ve postüral salınım indeksi değerlendirilmiştir. Tartışma: Hastaların yaş ortalaması 60.72± 7.05 (48-76) yıldı. Olguların gravite değişikliğine uyum süresi ile yaşları arasında pozitif ilişki saptanmıştır. (r:0,445) Ayrıca gravite değişikliği uyum süresi ile vücut kitle indeksi ölçümleri arasında anlamlı pozitif ilişkinin olduğu görülmüştür (r:0,429). Hiperglisemi parametreleri (HbA1c ve açlık kan glikozu) ile hareket ölçümleri arasında ilişki saptanmamıştır. Sonuçlar: Hipergliseminin hareket sistemi ile direkt ilişkisi gözlenmemiştir. Anahtar Kelimeler:Diyabetik nöropati, hareket, biyosensörler Çıkar çatışması: Yazarların çıkar çatışması bulunmamaktadır. Abstract Objective: Molecular diagnostics lies at the milestones of modern-day biochemistry. The identification of genetic and proteomic targets and patterns relies on the proper choice of detection methods, a combination of biochemical recognition architecture and signal processing. DNA-chip technology, by placing multiple probes on a single flats surface, allows for the storage of a tremendous amount of information and massively parallel detection of target species. The aim of this study, developing a new fast DNA-chip procedure for the detection of point mutations. Materials-Method: For this study, quartz crystal (qcm) electrodes surface modified with Poly Hema-Mac nanopolymer than, DNA-probes performed schiff base with the nanopolymer. Samples of genomic DNA were extracted from venous blood leukocytes and than, Arms (Amplification-Refractory Mutation System) method was used for creating amplicons. The amplicons were detected by using qcm DNAchip. We compare the results with gel electrophoresis. Results: The samples of wild type, α-thalassemia heterozygote, and homozygote samples PCR products were applied on the genosensor. When hybridization occurs on the electrode surface, quartz crystals frequency changes depend on the length of the probes. In this way, we can recognize the mutation type. Conclusıon: DNA-chip was evaluated for multiplex recognization of αthalassemia mutations. This nanopolymer based piezoelectric DNAchip can be using an alternative technique for the determination of αthalassemia mutations because it has more advantageous. For example, when this genosensor compared with conventional methods, it is faster, cheaper, more specific and less hazardous exposure.
doi:10.1515/tjb-2019-44s106 fatcat:bwjr2rbi5fetlenmztd6gg26qy