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Mono-and tri-ethyleneglycol aminooxy derivatives were reacted with levulinic acid, protected with dimethoxytrityl and immobilized on long-chain alkylamine controlled-pore glass (LCAA-CPG) solid support. The resulting solid supports were used to synthesize 3'aminooxy oligonucleotides having a shorter or longer spacer between the oligonucleotide and the aminooxy function. After completion of oligonucleotide synthesis, a first-step mild ammonia treatment was applied to remove the oligonucleotidedoi:10.1021/acs.joc.9b00848.s001 fatcat:ncpnql5uqnbefalgcfumvhouym