Egg yolk-free Baird-Parker medium for the accelerated enumeration of foodborne Staphylococcus aureus

R V Lachica
1984 Applied and Environmental Microbiology  
A simplified procedure is described for the accelerated enumeration of foodborne Staphylococcus aureus. This involves the replacement of egg yolk in the Baird-Parker medium with Tween 80 and MgCl2. These compounds, along with pyruvate, allow the recovery of stressed cells of S. aureus on a medium which contains potassium tellurite, LiCl, and glycine as selective agents. Black colonies are identified as S. aureus by the simplified thermonuclease test. The egg yolk (EY)-tellurite-pyruvate medium
more » ... f Baird-Parker (BP) (3) is generally considered superior to other media for the enumeration of foodborne Staphylococcus aureus (2, 5, 11, 12, 17, 18) . It is notable for its ability to VOL. 48, 1984 on March 23, 2020 by guest http://aem.asm.org/ Downloaded from
doi:10.1128/aem.48.4.870-871.1984 fatcat:4nufzac2kbhzdeb3tjwmjdzyoy