NADP +-specific Isocitrate Dehydrogenase from Bombyx mori, Order of Substrate Addition and Product Release

Fumio Miake, Takao Torikata, Katsumi Koga, Katsuya Hayashi
1977 Journal of the Faculty of Agriculture Kyushu University  
The kinetics of the reaction of NADP+-specific isocitrate dehydrogenase was examined by using the enzyme purified from the pupae of silkworm, Bombyx mori. The binding of the individual substrates, isocitrate and NADP+, depended upon each other, and was competitive with the reaction products, CO" cu-ketoglutarate and NADPH. The mechanism appears to be of a rapid equilibrium random type. These findings were very similar to those reported for NADP+-specific isocitrate dehydrogenases isolated from
more » ... everal sources. * Abbreviations: NADP+, nicotinamide-adenine dinucleotide phosphate, oxidized form; NADPH. as above, reduced form ; NADP-ICDH, NADP+-specific isocitrate dehydrogenase ; cuKG, a-ketoglutarate 83
doi:10.5109/23653 fatcat:mdwxiunmbvekhnlfjbm6qpoqpi