A pseudomolecule assembly of the Rocky Mountain elk genome

Rick E Masonbrink, David Alt, Darrell O Bayles, Paola Boggiatto, William Edwards, Fred Tatum, Jeffrey Williams, Jennifer Wilson-Welder, Aleksey Zimin, Andrew Severin, Steven Olsen
2021 PLoS ONE  
Rocky Mountain elk (Cervus canadensis) populations have significant economic implications to the cattle industry, as they are a major reservoir for Brucella abortus in the Greater Yellowstone area. Vaccination attempts against intracellular bacterial diseases in elk populations have not been successful due to a negligible adaptive cellular immune response. A lack of genomic resources has impeded attempts to better understand why vaccination does not induce protective immunity. To overcome this
more » ... imitation, PacBio, Illumina, and Hi-C sequencing with a total of 686-fold coverage was used to assemble the elk genome into 35 pseudomolecules. A robust gene annotation was generated resulting in 18,013 gene models and 33,422 mRNAs. The accuracy of the assembly was assessed using synteny to the red deer and cattle genomes identifying several chromosomal rearrangements, fusions and fissions. Because this genome assembly and annotation provide a foundation for genome-enabled exploration of Cervus species, we demonstrate its utility by exploring the conservation of immune system-related genes. We conclude by comparing cattle immune system-related genes to the elk genome, revealing eight putative gene losses in elk.
doi:10.1371/journal.pone.0249899 pmid:33909645 pmcid:PMC8081196 fatcat:dqaa66tzw5cztkyyb5qmrnkoia