Isolation of hematopoietic progenitors. An approach to two different immunomagnetic methods at the lab scale

J. A. Cancelas, S. Querol, G. Martín-Henao, C. Canals, C. Azqueta, J. Petriz, J. Inglés-Esteve, B. Amill, J. García
1996 Pure and Applied Chemistry  
The hematopoietic progenitors are a pool of cells which share the expression of a membrane glycoprotein: CD34. These cells are present in low frequencies in umbilical cord blood, adult peripheral blood and bone marrow (respectively, 0.1-0.6%, 0.01-0.1% and 0.8-4% of nucleated cells). The suitability of high-affinity monoclonal antibodies against the CD34 molecule, the necessity of rapid, sterile isolation methods and the chance of CD34-negative cell tumor purging in autologous stem cell
more » ... ntation or T-cell depletion in allogeneic transplantation has favoured a rapid development of different technical approaches for CD34+ cell selection. We can distinguish two different approaches to the CD34+ cell selection techniques: a) immunomagnetic which include a direct or indirect linkage between CD34+ cells and immunomagnetic particles, and b) immunoadsorption of CD34+ cells to an antibody-precoated plastic or avidinized poliacrilamide surfaces. All these techniques achieve variable yields between 30-70% and median purities ranging from 50-95%. An approach to the positive selection of CD34+ cells by two different indirect immunomagnetic methods is given.
doi:10.1351/pac199668101897 fatcat:fbxa7emf6vfadieetp6vmppyjq