Berechnung von Hydrolyse-Korrekturfaktoren am Beispiel der Zuckeranalyse nicht-dialysierbarer Magensaftkomponenten / Calculation of Hydrolysis Correction Factors for the Analysis of Bound Sugars and its Application to Gastric Juice

Th. Gheorghiu, K. Oette, V. Baumann
1970 Zeitschrift für Naturforschung. B, A journal of chemical sciences  
Different conditions for the acid hydrolysis of carbohydrate-containing substances, e. g. glycoproteins or mucopolysaccharides of body fluids were tested. The errors arising during quantitative analysis of hydrolysate components are discussed. Hydrolysis correction factors can be used in order to obtain the original sugar composition of the specimen. The authors tried to develop a procedure generally appliable to the calculation of such factors as a function of release and decomposition of
more » ... s under standard acid hydrolysis conditions (3 ɴ HCl for 3 h at 100 °C, sealed tubes, N2). The experiments were performed with lyophilisates of non-dialyzable substances of gastric juice and with the respective free sugars. Decomposition rates of sugars in the presence of a corresponding hydrolysate of juice proteins were studied under various conditions; the rate curves showed a nearly logarithmical evolution, their slope expressing the acid resistance of the sugar in the given case. Basing on these rates and on the concentration curves of sugars in the native juice hydrolysed under the same conditions, an empirical formula was developed whose different mathematical aspects are subsequently analyzed.
doi:10.1515/znb-1970-0811 fatcat:tnn5jhjj7ngcfixufqn2jrmtoi