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Zucchini yellow mosaic virus (ZYMV) is one of the most destructive virus of cucurbits and found throughout the cucurbits growing areas of Pothowar region of Pakistan. Overall 300 samples were randomly collected from 40 cucumber growing fields from all over the pothowar region during two consecutive years (2015-16 and 2016-17). All the samples were screened by Double Antibody Sandwich-Enzyme Linked Immunosorbant Assay (DAS-ELISA). Relative incidence of ZYMV recorded in two consecutive years wasdoi:10.19045/bspab.2019.80148 fatcat:twcjfsmj25cm5kaxt65cacmatu