EcoRI cleavage sites in the argECBH region of the Escherichia coli chromosome

E A Devine, M C Moran, P J Jederlinic, A J Mazaitis, H J Vogel
1977 Journal of Bacteriology  
The EcoRI cleavage of deoxyribonucleic acids (DNAs) from Xdarg phages, carrying argECBH, has been examined. The phages are derived from the heatinducible, lysis-defective strain Xyl99, and their bacterial DNA, including arg-ECBH, is derived from Escherichia coli K-12. Such cleavage of the phage DNAs, in each case, produces the D, E, and F segments of A. Additionally, these DNAs yield segments, ordered from left to right, of length (in kilobases [kb]) determined by electron microscopy and 0.7%
more » ... arose slab gel electrophoresis, as follows: Xdargl3 (ppc argECBH bfe), 13.9, 2.8, 1.5, and 5.6; Xdargl4 (ppc argECBH), 3.0, 2.0, 17.3, and 6.2; and Xdarg23 (argECBH), 18.4 and 6.2. For Xdargl3 suplO2 DNA, the segment analogous to the 13.9-kb segment measures 12.2 kb. The direction from left to right corresponds to the clockwise orientation of the E. coli genetic map. The EcoRI segments define five cleavage sites near the arg region of the E. coli chromosome. For each of the DNAs, the arg genes occur on the largest segment produced. The 17.3-kb segment, being entirely bacterial, represents the argECBH-bearing EcoRI segment of the E. coli chromosome. The location of the arg genes was demonstrated electron microscopically in heteroduplex experiments.
doi:10.1128/jb.129.2.1072-1077.1977 fatcat:p64zjxlzxzbsrmz4shpndjalfq