EVALUATION OF THE GENETIC VARIABILITY CORRELATED WITH MULTILEAFLET TRAIT IN ALFALFA

Sorina Popescu, Oana-Maria Boldura, Sorin Ciulca
2016 AgroLife Scientific Journal   unpublished
The multileaflet trait on alfalfa determine high biomass and increased protein content, therefore the understanding of the metabolic pathway controlling this trait it is of great importance. Different mechanisms were already described, but none of them give a complete explanation for this phenotype. The aim of our work was to evaluate the genetic variability for 8 different plants, both tri and multileaflet ones, originated from MF 42-96 alfalfa synthetic line developed by ICDA Fundulea and
more » ... -type cultivated alfalfa, based on ISSR (Inter Simple Sequence Repeat) markers. Eight ISSR markers were selected from a collection of 20 primers, due to their high polymorphism among the analyzed samples. All the primers generated 157 fragments of which 122 (77.7%) were polymorphic. The evaluation with the UPGMA (unweighted pair-group method) clustering method generated information regarding variability and the dendrogram. The determined similarities coefficients range between 0.44 and 0.64, pointing out a medium variability between samples, with no correlation between phenotypes. In the same clusters were present both tri and multileaflet plants. However, seven polymorphic fragments were identified, present only in multileaflet individuals fingerprints, but absent for all the trileaflet ones. These fragments could be possible candidates to develop a microsatelite markers linked with the multileaflet phenotype on alfalfa.
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