Water-soluble Component in Dried Chrysanthemum Flower Stimulates Tumor Necrosis Factor-.ALPHA. Production by Mouse Macrophage-like Cell Line RAW264.7
Food science and technology research
To clarify immunoregulatory activity of "Shiranui Himekiku" (Chrysanthemum indicamῌErigeron annus), we examined the e#ect on tumor necrosis factor a (TNF-a) production by mouse macrophage-like cell line RAW,0..1. The dried chrysanthemum flower (CF) petals were extracted with water, and the cells were cultured in the presence of the extract. CF extract significantly enhanced TNF-a production of the cells by the dose-dependent manner. Diluted CF solution did not significantly a#ect the cell
... #ect the cell number and viability ; however, non-diluted solution suppressed the cell proliferation and decreased the cell viability. Heating CF extract at +**῍C for -* min enhanced the activity of water extract markedly, and freeze-thawing only moderately. The TNF-a production enhancing activity of the extract was observed withinh. These results suggest that the TNF-a production enhancing activity of CF extract can be recovered e$ciently by hot water extraction and preserved stably by freezing.