Water-soluble Component in Dried Chrysanthemum Flower Stimulates Tumor Necrosis Factor-.ALPHA. Production by Mouse Macrophage-like Cell Line RAW264.7

Satomi KOBAYASHI, Naoto OGAWA, Yoshinori FUJIMURA, Hirofumi TACHIBANA, Koji YAMADA
2006 Food science and technology research  
To clarify immunoregulatory activity of "Shiranui Himekiku" (Chrysanthemum indicamῌErigeron annus), we examined the e#ect on tumor necrosis factor a (TNF-a) production by mouse macrophage-like cell line RAW,0..1. The dried chrysanthemum flower (CF) petals were extracted with water, and the cells were cultured in the presence of the extract. CF extract significantly enhanced TNF-a production of the cells by the dose-dependent manner. Diluted CF solution did not significantly a#ect the cell
more » ... #ect the cell number and viability ; however, non-diluted solution suppressed the cell proliferation and decreased the cell viability. Heating CF extract at +**῍C for -* min enhanced the activity of water extract markedly, and freeze-thawing only moderately. The TNF-a production enhancing activity of the extract was observed withinh. These results suggest that the TNF-a production enhancing activity of CF extract can be recovered e$ciently by hot water extraction and preserved stably by freezing.
doi:10.3136/fstr.12.144 fatcat:opye5apr55dyrmgrp5dwnm5zl4