Probiotic Properties of Lactic Acid Bacteria isolated from Feces and Kimchi
베트남인 분변 및 김치로부터 분리된 유산균의 프로바이오틱스 기능성 연구

Hyun Su Shin, Sung Ho Yoo, Jin Ah Jang, Ji Young Won, Cheol Hyun Kim
2017 Journal of Milk Science and Biotechnology  
The purpose of this study was to investigate the probiotic properties and antioxidant capacity of lactic acid bacteria isolated from Vietnamese feces and the Korean traditional food kimchi. Six isolated strains were identified as Lactobacillus sp. by 16S rRNA sequencing. All strains showed good resistance to low pH (1.5, 2.0, and 3.0) and 0.3% oxgall bile acids. Culture filtrates from the six strains showed various antioxidant effects, including DPPH, ABTS, reducing power, and metal chelating
more » ... e 2+ ) activities. Two of the six Lactobacillus strains showed potential probiotic activity. Heat resistance and adhesion assays were conducted by mixing the selected strains, Lactobacillus acidophilus V4, Lactobacillus plantarum V7, and Lactobacillus paracasei DK121 isolated from kimchi. The results showed that the heat resistance of these strains was similar to that of a commercial strain, L. plantarum LP. In addition, a mucin attachment assay using the mixture of selected strains (V4, V7, and DK121) showed high binding activity to the mucous layer. In conclusion, a mixture of V4, V7, and DK121 shows promising probiotic activity and may be useful for the development of health-related products. Keywords probiotics, lactic acid bacteria, Vietnamese, antioxidant, mucin adhesion 서 론 생균제(Probiotic)란 살아있는 미생물 균체를 섭취함으로써 미생물이 분비하는 효소, 유기산, 비타민 및 무독성 항균물질 등에 의한 장내 균총의 정상화는 물론 장질환 치료를 통해 신체기능 개선을 목적 으로 생산된 제품을 말한다(Rivero-Perez et al., 2008). 생균제의 종류로는 유산균(Lactobacillus)과 진균(곰팡이, Aspergillus, 효모, Saccharomyces) 등이 일반적으로 널리 알려져 있는데 이 중 가장 대표적인 것으로 Lactobacillus(Lee et al., 2008)를 들 수 있다. 젖산균은 복잡한 필요 영양 조건을 가지고 있으며, 프로바이오틱스 또는 발효식품에 이용된다. Lactobacillus 및 Bifi-dobacteria와 같은 유산균은 한 가지 이상의 종만으로도 장내 균총에 직접 영향을 미칠 뿐만 아니라, 면역조절, 장투과성, 생체활성 또는 신진조절 대사산물을 생성함으로써 다른 장기에 영향을 미친다 (Khanizadeh et al.
doi:10.22424/jmsb.2017.35.4.255 fatcat:o4nwuq6izbcnvmruvskatt5qca