UV-SPECTROMETRIC AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF PENTOXIFYLLINE IN WORKPLACE AIR

V Christova-Bagdassarian, T Angelov, M Atanassova
2007 Journal of the University of Chemical Technology and Metallurgy   unpublished
Pentoxifylline is a pharmaceutical drug used to eliminate or to reduce disturbances in the peripheral blood circulation. Two methods with the same sampling and sample preparation for determination of pentoxifylline in workplace air are developed. Air sampling at workplace is made by means of perchlorovinyl filters (FPP). A twice methanol extraction for 20 minutes from FPP-filters is used. A simple UV-spectrometric procedure is described. The average recovery is 85.3 – 95.03 %. The method is not
more » ... . The method is not selective. A selective reversed-phase liquid chromatographic method for the determination of pentoxifylline in the air of working environment is developed, too. Aliquot (50 µl) of extract is injected and then separated on a reversed phase RP LiChrosorb C 18 Perkin Elmer column (5 µm, 250 mm x 4.6 mm i.d). The mobile phase is acetate buffer (pH 4.3): acetonitrile 3:7 (v/v) with flow rate 1.0 cm 3 min-1. The UV-detection at 275 nm is used. The average recovery is 86.8 – 94.1 %.
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