Biosensor Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92, A. P. C. Road, Kolkata-700 009, India E-mail : psarkar@cubmb.ernet.in Fax :91-33-4852976 Manuscript received 26 June 2001, revised 13 December 2001, accepted 3 April 2002 This paper describes a new technique of immobilization of enzyme on an amperometric biosensor to enhance response as well as stability. Glucose oxidase (GOD) was chosen as the model enzyme and cellulose acetate butyrate (CAB) as the

more »

... poly­mer for encapsulation. The encapsulation was achieved by multiple emulsion technique. GOD was also immobilized by cross-linking with glutaradehyde directly on the surface of the electrode and results from a solution of 2 mM glucose were com­pared. The microencapsulated GOD electrodes proved to be superior to cross-linked GOD electrodes both with respect to response and stability.