Preparation and characterization of highly pure fractions of outer membrane, cytoplasmic and intracytoplasmic membranes from Ectothiorhodospira mobilis

T. DITANDY, J. F. IMHOFF
1993 Journal of General Microbiology  
Outer membrane (OM), cytoplasmic membrane (CM) and intracytoplasmic membranes (ICM) from the halophilic phototrophic purple sulphur bacterium Ectothiorhodospira mobilis 9903 were purified and characterized. The three membrane fractions were significantly different in regard to protein profiles on SDS-PAGE, and to the composition of amino acids, fatty acids and lipids. The presence of lipoproteins, the occurrence of lyso-phosphatidylethanolamine and an increased content of saturated and
more » ... urated and short-chain fatty acids are characteristic properties of the OM. CM and ICM fractions are different on the basis of buoyant density, of protein profiles and amino acid composition, and due to the presence of succinate dehydrogenase activity in CM. In addition, CM and ICM showed significant differences in pigment content and absorption spectra. 0001-7613 0 1993 SGM Downloaded from www.microbiologyresearch.org by IP: 134.245.215.185 On: Fri
doi:10.1099/00221287-139-1-111 fatcat:t5bztldsuzaqrl4gsimpmr2o3i