using a modified hunlan tumor clon 〔 〕 genic assay . Peripheral blo 〔, d m ( monuclear cel ] s ( PBMC ) were used as feeder cells ( a mixture of 5 x lO4 nlonocytes 〆 dish and 5 × ユ〔 } 51ymphocytes / disl 、 obtained from healthy donors ) . KK − 47, TCCSUP and T24 , all derived fr 〔 〕m hunnan transitional cell carcinolnas Qf the urinary bladder , were incubated c . ol コtilluoUs ] 〉 , with vurious conce [ ltr・ ations of rG ・ CSF rangil19 fr()nl O. lnghnl t〔} 1〔 [ ng / ml both with and without PBMC

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... for 7 t〔 〕 21 days. rG . CSF in the upper Iayer at c 〔 mcelltrations ranging from ( ) 、 lng / ml to 10ng/ml stimulated the colon } ・ f⊂)rmation c)f all the cancer cell Iines tested in the absence of PBMC ill the feeder layer , whereas cells with PBMC ill the feeder layer were significantly stimulated more than th⊂) se without PBMC ill the feeder] ayer ( p < 0, 05)し1p to a certain concentratiQn which varied frQm cell line to N 工 工 一 Eleotronio Library